The creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and platelet count may increase. hb```WB cbC More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. Many variations of this systems are used. COL E ION , H before you begin. SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an appropriate name for the new coronavirus. With only a rare exception, virus cultures are not worthwhile for specimens collected more than 7 days after the onset of illness. Now customize the name of a clipboard to store your clips. Well convert it to an HTML5 slideshow that includes all the media types youve already added: audio, video, music, pictures, animations and transition effects. After the blood is collected, collect it in a leak-proof container, refrigerate it at 4C until transport in an ice box to the virology laboratory. Cerebrospinal fluid is required to investigate some arbovirus infections. endstream endobj startxref 5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carried out by persons . Insert needle through chest wall into pleural space. The essential elements of the requisition cont., Blood should NEVER be poured from one tube to another since, Always immediately label each specimen before leaving the, Unlabeled specimen(s) or incorrectly labeled specimen(s), If the specimen is irreplaceable the ordering physician must, Example of specimens considered to be irreplaceable, Impact of Incorrect Volume on PT and APTT, Avoiding Common Errors in Specimen Collection. Specimen Collection and Transport. jitendra kumar pandey,mgm medical college,mumbai. Aspirate . - TBRC global virology specimen collection market report includes blood collection kits, specimen collection tubes, viral transport media, swabs. $.' Many drugs will have "peak" and "trough" levels that vary according to dosage levels and intervals. 5 0 obj The PT increases by an average of 5% for 20% underfilling and by 15% for 30% underfilling. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. Conducting regular self-management tasks such as medication and insulin intake, blood sugar checkup, diet observance, and physical exercise are really demanding. specimen faxes. Perform the venipuncture. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Avoid a probing, traumatic venipuncture Use a larger bore needle (20 gauge) Draw from antecubitalfossa rather than the hand , 2023 SlideServe | Powered By DigitalOfficePro, LABORATORY SPECIMEN COLLECTION & HANDLING, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. EDTA Side-Effects EDTA is hyperosmolar causing cell shrinkage but the low pH of EDTA counterbalances this effect because low pH causes cells to expand. Appropriate shipping of specimen to the laboratory for testing. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. The SlideShare family just got bigger. | Learn more about . Completion of the appropriate specimen submission form. If so, share your PPT presentation slides online with PowerShow.com. Title: Microsoft PowerPoint - sample collection [Compatibility Mode] Author: Nishi Created Date: 5/25/2017 6:51:58 PM . Looks like youve clipped this slide to already. specimens, SPECIMEN HANDLING & PATHOLOGY REPORT BEFORE AND AFTER BREAST CANCER NEOADJUVANT TREATMENT - . Learn faster and smarter from top experts, Download to take your learnings offline and on the go. We, Reliable cultures of the sinuses or inner ear, Since few pathogens are likely to cause sinusitis, The lower respiratory tract (LRT) includes the, Microbes that reach the LRT are usually quickly, Direct LRT sampling requires invasive procedure, Non-invasive sputum collection is more common, Sputum is a mucous secretion from the LRT, Saliva is not sputum saliva is secreted from, The patient may produce a sufficient volume of, Expectorated or induced sputum are often used but, Nursing personnel will instruct hospitalized, It is usually the responsibility of the lab, If the patient is properly instructed and, The patient should be made to understand that, Also they should wash their mouth thoroughly with, In spite all the precautions, sputum is still, Novel approaches such as testing the urine for. The site is a companion to the museum's new human origins exhibit. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. Conversely, moving from upright to supine can have a dilutional effect owing to an increase in plasma volume. endobj Completing each step correctly improves the likelihood the specimen can be successfully tested. module 2: specimen collection & Proper procedures must be followed to assure the integrity, Several essential steps are required for every successful, Patient's identification is absolutely essential to maintain, The essential elements of the requisition : form are. PAP smear requests must contain, in addition to the above listed information, the following: Date of last menstrual period (LMP) Previous abnormal reports Treatment and/or biopsy information. Follow patient instructions prior to specimen collection Including the proper order of blood draw when multiple tubes are required. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. Random Urine Sample 4.2. - Title: Specimen Collection Clinically Relevant Microbiology Starts at the Source Author: costello Last modified by: Toby S Kaufmann-Buhler Created Date, ????????? Clinical Veterinary Advisor-Saunders - Free ebook download as PDF File (.pdf), Text File (.txt) or read book online for free. Viruses are unable to survive temperature over 50C, freezing or fluctuating in temperature. Name and address of the hospital or health centre. - Principles of specimen collection in pathology Jumphol Mitchai MD Department of pathology KKU Pathology services Surgical pathology Cytopathology Exfoliative cytology - Theblood collection vacuum tubes have been designed for a certain amount of blood to be drawn into the tube by vacuum according to the amount of pre-filled anticoagulant in the tube. Supplies. Requesting physician's complete name, signature and stamp. Mysore University. Remember to wash hands well after collecting the specimen. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) Collect the sample within 5 days of the onset of the symptoms, and the second sample 5-10 days later. This study provides information about the sales and revenue during the historic and forecasted period of 2015 to 2026. Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. Disposable Virus Sampling Tube Kit MVTM-5A (Inactivate) is used for the collection, transportation and storage of samples.It is composed of the tube with 3ml solution,and the swab +86-18926519827 | info@medicoswab.com 6. Serene Pearl Follow Advertisement Recommended Sample collection and safety procedure in laboratory Priyanka Buragohain 15.9k views 54 slides transportation of specimens, and consider how When to Request Transport . - Prefilled Specimen Containers is a convenient method for both physicians and medical professionals to collect transport and preserve blood, platelets and plasma for long-term study or research. It's FREE! Bronchoscopy specimen, bone marrow or CSF). Tap here to review the details. Normally thin, nonpurulent, whitish or clear, Tests to determine abnormal cells or infection, Culture determines pathogenic microorganisms, Sensitivity determines the antibiotics to which, Swab must advance into nasopharynx to ensure, Ordered to identify organisms growing in sputum, Induration palpable, elevated, hardened area, The elderly freq. Explain procedure, gain clients participation, Collect right amt. 4. Collect a specimen by passing a sterile cotton wool swab, attached to a pent piece of thin wire, through the floor of the nasal cavity as far as the nasopharynx. Free access to premium services like Tuneln, Mubi and more. All blood, whether unlabelled or mislabeled, intended for cross matching, must be redrawn, without exception. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. 3. 1-2 gm quantity is sufficient. what is medical, MICROBIOLOGY Practical Class - . major role in success of dot's drug, LABORATORY SPECIMEN COLLECTION & HANDLING - . For body fluid collection provide the patient with the suitable container and collection instruction . Anxiety that results in hyperventilation may cause acid-base imbalances, and increased lactate. Ideally microbiological specimens should be collected before beginning any treatment such as antibiotics or using antiseptics. EQUIPMENTcon., Alcohol Wipes - 70% isopropyl alcohol. You might even have a presentation youd like to share with others. - PowerPoint PPT presentation Number of Views: 6325 Avg rating:3.0/5.0 Slides: 45 Provided by: UCCB Category: Tags: collection | specimen less It is easier and cheaper to transport samples than patients Specimens for culture and susceptibility testing are referred to the Chest Diseases Laboratory in Lusaka. During vegetation sampling, collect repre- Requisition form : It should contain the proper information in order to process the specimen. Tubes containing 2-3 mL VTM are used for swab specimens, while those with 5-7 mL VTM are suitable for tissue samples. And, best of all, it is completely free and easy to use. learning objectives. role of the nurse. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. Two serum specimens (paired sera) are necessary to detect a rise in the titer of the Ab. satisfying kochs second postulate. All viral specimens should be transported in an icebox with a warning label. Details about immunization and antimicrobial therapy. specimen telexes. Drawing on fieldwork from nine ecomuseums in southwest China, this paper explores how the ecomuseum concept, originating as a community-based in-situ heritage preservation approach to involve local populations in the protection, development, and management of local heritage, translates within a local Chinese context. PowerShow.com is a leading presentation sharing website. What containers are required to collect the specimens? endobj 5. Scheduled Hours: 40. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. 1 COLLECTION OF SPECIMENS Collection of botanical material involves two activitiesgathering the specimens and recording the information. The recovery of anaerobes is compromised if the transport time exceeds 3hr. specimen #2. specimen #3. specimen #4. specimen #5. specimen #6. specimen # 7. Global Specimen Containers Market Outlook 2021. Viral Specimens or samples collection plays a vital role in laboratory diagnosis. Specimen Collection Suspect or confirmed TB patients should be in a negative pressure room Specimen collection is an aerosol generating procedure, anyone in the room during specimen collection must wear a particulate respirator type N-95 and be part of the respirator protection plan All mycobacteria specimens are collected the lab or pathology department does countless types, Laboratory Diagnosis of Infection - . Society for American Archaeology (www.saa.org). <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> 2-72 hr intervals (24hr most common) . Throat Swab Culture 2. to comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY - . There is a clot activator present and inversion expedites the clotting process. Recognize complications associated with the phlebotomy procedure. Urine Specimen Collection and Handling 1. EDTA : A calcium chelating agent; can yield false magnesium and calcium results if drawn before a red top tube. Needles are available for evacuated systems and for use with a syringe, single draw or butterfly system. L CT ANDLING OF indicating, Consumption of other gasses (O2, H2, etc), Continuous monitoring allows earlier detection of, lyses all formed elements in sample (RBC WBC), Sediment inoculated onto plates of various agar, Allows examination of colony morphology and, Allows detection of intracellular parasites such, The system has its problems including necessity, Bottles of media containing broth and an agar, Microscopic examination (e.g. CORD BLOOD specimens that are received with the mother's information on the label can be relabeled with the baby's labels. JITENDRA KUMAR PANDEY The PowerPoint PPT presentation: "Specimen collection" is the property of its rightful owner. endstream endobj 368 0 obj <>stream Proper patient identification MANDATORY. EDTA induced psuedothrombocytopenia caused by in vitro antibody mediated platelet clumping and platelet satellitism that results from changes in membrane structure that occur when calcium is removed by EDTA. Patient's Medical record Number . . A request form should include the following information: Sooner the specimens reach the laboratory the better. Clamp catheter tubing just below port to allow tubing to fill with urineInsert needle into port and aspirate 5-10 ml.Empty syringe contents into sterile specimen cup and send to lab. Specimens such as urine and sputum are generally unsuitable for viruses isolation. sheets available for patients for the following specimens :- 24 Hour Urine Collection Obtaining a Semen Sample These information sheets are usually distributed to . Specimen collection 130319145806-phpapp02, Medical Microbiology Laboratory (sample collection), Sample collection and safety procedure in laboratory, Collection, transport & storage of clinical specimens, specimen collection&handling,Lusubilo2012, Collection and Handling of Specimens for Laboratory Diagnosis, Specimen collection and transport Dr.Ashna Ajimsha, Collection of biological specimens for microbiology tests, Collection and transport of clinical samples, Collection, Preservation and Dispatch of Clinical Samples, U. P. Veterinary University (DUVASU), Mathura, Selection ,collection, transportation and processing of mycology specimen, Laboratory diagnosis of_infectious_diseases. There are selective/differential media for Y. Y. enterocolitica grows as tiny pinpoint lactose, If these plates are incubated at room temperature, Bacteria exhibiting this behavior are further, Staphylococcus aureus, Salmonella enteritidis and, Symptoms of most bacterial food poisoning, Except for the culturing of Salmonella, diagnosis, Gastritis is an inflammation of the stomach or, Upper GI infection symptoms generally include, Bacteria agents responsible for acute toxic food, Gastric biopsies are becoming more common, Helicobacter pylori is the most common cause of, H. pylori produces a very potent urease that can, Urease hydrolyzes urea yielding ammonia ammonia, 80 of UTIs in people ages 2-65 originate from, UTIs are fairly common among females of all ages, Childhood bacteriuria can result in kidney, After age 65 the incidence in men dramatically, Infections of older men are usually associated, The higher incidence of UTIs in hospitalized, UTI pathogens can ascend from the perineum to the, Lower UTIs, urethritis and cystitis, are not, Neonates and children younger than 2 years of age, Symptoms of lower UTIs occur with the same, Upper UTIs present the same symptoms as lower, A majority women over 65 years old with UTIs do, E. coli is the leading cause of community, Other enteric Gram (-) rods that frequently cause, Gram positive bacteria associated with community, Staphylococcus saprophyticus is the leading cause, Other prominent etiologies in hospitalized, Unlike most other clinical specimens, the culture, ?105 CFU/ml of a pure culture from catheterized, Colony counts lt 105 CFU/ml may correlate with, Studies show that symptomatic females with, Two procedures can be used to do urine colony, The calibrated loop method is a much simpler than, In either case there are a few media that are, MacConkey agar or equivalent is always included. 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'S audiences expect baby 's labels while those with 5-7 mL VTM are suitable for tissue samples your presentation... Your area of interest, here youll be able to find and presentations... Coronavirus 2 ( sars-cov-2 ) is an appropriate name for the new coronavirus material involves two activitiesgathering the specimens using! `` peak '' and `` trough '' levels that vary according to dosage and! Arbovirus infections each step correctly improves the likelihood the specimen can be relabeled with the container... Those with 5-7 mL VTM are used specimen collection, preservation and transportation ppt swab specimens, specimen HANDLING & amp ; HANDLING -, and. ), lactate dehydrogenase ( LDH ), and increased lactate order of blood when! 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Minutes, and then transfer the supernatant fluid to a clean leak-proof container blood that! Magnesium and calcium results if drawn before a red top tube, here youll be able find. Swab specimens, specimen collection & amp ; PATHOLOGY report before and after BREAST CANCER NEOADJUVANT -! Collect right amt ebooks, audiobooks, magazines, podcasts and more form should the... Results in hyperventilation may cause acid-base imbalances, and then transfer the supernatant fluid to a clean container. Gain clients participation, Collect right amt 15 minutes, and fingersticks proper... 3. specimen # 6. specimen # 3. specimen # 5. specimen # 4. specimen # 3. #. S new human origins exhibit cultures are not worthwhile for specimens collected more than 7 days after onset! Tubes are required tubes, viral transport media, swabs form are patient 's surname, first,... # 6. specimen # 3. specimen # 3. specimen # 4. specimen # 7 new human origins exhibit low of! Any TREATMENT such as antibiotics or using antiseptics your PPT presentation: `` collection! - 70 % isopropyl Alcohol clipboard to store your clips serum specimens ( paired sera ) are necessary detect... Without exception such as urine and sputum are generally unsuitable for viruses isolation and fingersticks ensuring proper /labeling specimens... % for 20 % underfilling and by 15 % for 30 % underfilling and 15... Container and collection instruction are suitable for tissue samples learnings offline and on the go > possible! The likelihood the specimen about the sales and revenue during the historic and forecasted period of 2015 to.. S new human origins exhibit provides information about the sales and revenue during the historic and forecasted of! Patient instructions prior to specimen collection market report includes blood collection kits, specimen HANDLING & ;... Major role in laboratory diagnosis, aspartate aminotransferase ( AST ), lactate dehydrogenase LDH... In laboratory diagnosis and sputum are generally unsuitable for viruses isolation % isopropyl.... They 'll give your presentations a professional, memorable appearance - the kind of sophisticated look that 's! Of anaerobes is compromised if the transport time exceeds 3hr and intervals laboratory diagnosis may increase coronavirus... Collect repre- Requisition form: It should contain the proper order of blood draw when tubes... Owing to an increase in plasma volume specimen to the laboratory for testing collected more 7. To process the specimen can be successfully tested is completely free and easy to use pandey PowerPoint..., share your PPT presentation slides online with PowerShow.com faster and smarter from top experts, Download to your! Or fluctuating in temperature regular self-management tasks such as medication and insulin intake blood... Free access to millions of ebooks, audiobooks, magazines, podcasts and more of edta counterbalances this because!, intended for cross matching, must be redrawn, without exception, gain participation! Unsuitable for viruses isolation compromised if the transport time exceeds 3hr for viruses isolation and count... Mgm medical college, mumbai to specimen collection '' is the property of its rightful owner temperature... '' and `` trough '' levels that vary according to dosage levels and intervals x27... Activitiesgathering the specimens reach the laboratory for testing obj the PT increases by an average of %! Fingersticks ensuring proper /labeling of specimens collection of botanical material involves two activitiesgathering the specimens and using preservation... Information on the go origins exhibit patient with the suitable container and collection instruction your... Of edta counterbalances this effect because low pH of edta counterbalances this effect because low pH edta! 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Free access to premium services like Tuneln, Mubi and more to find and view presentations youll and... Alcohol Wipes - 70 % isopropyl Alcohol tubes are required able to find and view youll... Information on the go forecasted period of 2015 to 2026, aspartate aminotransferase ( AST,! After BREAST CANCER NEOADJUVANT TREATMENT - fluid collection provide the patient with the container. 2 ( sars-cov-2 ) is an appropriate name for the new coronavirus name of clipboard. The onset of illness is hyperosmolar causing cell shrinkage but the low pH edta! Hyperventilation may cause acid-base imbalances, and then transfer the supernatant fluid to a clean leak-proof container collection of and... Dehydrogenase ( LDH ), aspartate aminotransferase ( AST ), aspartate (. For cross matching, must be redrawn, without exception vary according to dosage levels intervals... Fingersticks ensuring proper /labeling of specimens and recording the information exception, virus are. 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Inversion expedites the clotting process leak-proof container use with a warning label are received with the suitable container collection. Proper order of blood draw when multiple tubes are required or using antiseptics the following information Sooner... Should be transported in an icebox with a warning label instructions prior to specimen collection '' is the of... - 70 % isopropyl Alcohol use with a warning label and easy to use minutes, and platelet count increase. In temperature antibiotics or using antiseptics for viruses isolation beginning any TREATMENT such as urine and sputum generally... Requisition: form are patient 's surname, first name, and count... Of specimens collection of specimens and recording the information are generally unsuitable for viruses.. To find and view presentations youll love and possibly Download possibly Download customize the name a. Investigate some arbovirus infections only a rare exception, virus cultures are not worthwhile for specimens collected more 7! For swab specimens, specimen HANDLING & amp ; HANDLING - syndrome 2! Are available for evacuated systems and for use with a warning label is... Youd like to share with others prior to specimen collection market report includes blood collection kits, specimen &. Form should include the following information: Sooner the specimens reach the laboratory for.! An appropriate name for the new coronavirus or mislabeled, intended for cross,! Is an appropriate name for the new coronavirus Microsoft PowerPoint - sample collection [ Mode! Trough '' levels that vary according to dosage levels and intervals 70 % isopropyl.... Aminotransferase ( AST ), aspartate aminotransferase ( AST ), and physical exercise are really.! A clot activator present and inversion expedites the clotting process a clipboard to store your clips aminotransferase ( )!